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Hello everyone and welcome back to our podcast the researchers round table.

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In this episode we will talk to Dr. Sam Amsberg a lecturer at University of San Francisco.

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Sam works on plant cell wall and he will share his passion for science.

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So for quite a long time when I was a really little kid I was really interested in science

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and ecology and pollution.

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And his journey to become a lecturer at University of San Francisco.

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I'd say it's been quite a steep learning curve so I was very fortunate I finished my PhD

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and lined up.

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He shares his experience during his early career research fellowship.

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I think your grant has to be focused, your proposal has to have, you can't.

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And how does Sam unwind himself?

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After weekend to do whatever I want to do, go and see friends, I've got allotment, I

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go and guard and take time.

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There is much more to learn from Sam's journey from undergrad to PhD, from postdoc to current

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lecturer.

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A good example of having work life balance because I feel like those sorts of things

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have to come from the top.

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If your PI works all the time it is always in.

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So stay tuned to learn more.

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Welcome to our show Sam, can you tell me about your current position in the Sheffield?

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Yes, so at Sheffield I'm a lecturer but I've just started that position in January so I

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was on an early career fellowship before that and when that rolled to the end I was lucky

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that Sheffield were recruiting so I applied for a lecturer job and just started that.

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So that's nice.

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So I will start this conversation with again like when it inspired you to come to science

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as a child or what time you realised I need to go for the science?

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So for quite a long time when I was a really little kid I was really interested in science

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and ecology and pollution, my parents were quite interested in that and then I sort of

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lost interest in it through my teenage years and towards the end of school I became really

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interested in biology.

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I had a really inspirational biology teacher so I applied for a biology degree at university

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so that's really my kind of origin story in science and then I really enjoyed my degree

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so that's when I decided I kind of want to stay in this area and keep doing that.

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So you did your master from Sheffield or?

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I didn't do a master so I did my degree at Manchester first in biology and then I transitioned

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to plant science and then I did a PhD in Sheffield and then went away a bit to work in Leeds

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and then I've been in Sheffield ever since.

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So I will start again with the PhD, what was the topic, what were you working on, how you

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come to like I need to go for this, you know scientists to work with?

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Well there's a mix of personal and professional reasons so we wanted to stay up in the region

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so Sheffield is very close to Manchester where I did my degree and my partner was working

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in Manchester at the time but also my third year undergraduate project supervisor knew

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my PhD supervisor in Sheffield well and so recommended the project because it was in

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a related area on kind of the biomechanics of plant cell walls and gave me a good reference

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so I applied for it and initially I didn't get it and then there was another project

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available and I got it on the second swing so it was a bit of luck but really I was just

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looking for plant science related projects that were interesting and there was a lot

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of different projects at the time that interested me but this one was about something related

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to my third year project so I felt I had a little bit to offer to it.

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So currently you're working on cell plants cell wall and all those things so during your

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PhD you did the same thing?

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Yes in a different system so I worked in my PhD on very specifically the kind of nano

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biomechanics of stomatal cell walls and then I've kept some of those techniques and taken

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them forward in my career but I've sort of broadened that out to look at cell walls in

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other contexts and kind of broadened that research strand.

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Oh great so during your postdoc because after your PhD I have seen your track like you

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moved there was no any gap like you have continued to float for you know PSD then postdoc then

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the discovery fellow.

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Yeah I've been very fortunate.

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So can you tell me briefly about like how is the journey from like a postdoc to early

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careers like a discovery fellow and all?

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I'd say it's been quite a steep learning curve so I was very fortunate I finished my PhD

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and lined up a job in Leeds with a postdoc with Joseline Venitas Alfonso who was then

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doctor is now professor and I was very fortunate that she's an incredibly supportive supervisor

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so she really encouraged me to develop my own ideas and apply for things and she provided

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me with a lot of support for writing the discovery fellowship application and I was still living

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in Sheffield at the time from my PhD so I had a vested interest in moving back to Sheffield

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and I really like the department at Sheffield they have really brilliant growth facilities

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and there's just a really big group of plant scientists there's a lot of people to bounce

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ideas off so I kind of wanted to be back in Sheffield and I just applied for it and it

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was a really long process to write it so I guess the first bit of advice would be start

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a year in advance of when you need it to start because it took a long time to go through

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the interviews and the reviews.

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Yeah it's my personal like a question like is it when you're applying for independent

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philosophy your profile really matter because sometimes you are leaving your PhD you don't

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have that much profile but we say like oh you need some paper for like large paper big

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paper something.

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There's a mix I would say yes obviously your profile does matter but I was not the most

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prolific PhD student I had I had a nice paper for my PhD and then in my postdoc we had a

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couple of small additional papers but I did not have a huge CV with loads of big impact

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nature science papers and loads of first author papers so I think you need to have something

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to show that you can drive a research project through to a good publication but field specific

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publications that are kind of lower impact I think are fine and you've then just got

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to write a really good good project that's really well articulated that sells the problem

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well and I think that's as long as your CV is good enough I think the project and how

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you present it and how you sell the idea is really more important.

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So it's like you can apply it's not matter that you should have a nature or something

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but I think the area of interest where you're applying for the post like you working you're

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you know when I see your work it seems like you have moved from but you haven't left that

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area of research and it seems like you have you know grip on those subject when you're

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writing people can see that but yeah this guy can bring something new.

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Yeah kind of build on your skill set if you start something completely different you have

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no track record on it even if you've got papers in a different field.

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Yeah so it's happened with me like I started with my map kind of signaling back in India

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I did in macroanalyzing assist then moved to glucose signaling then go to AVS signaling

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and later now I'm doing the simulation so somehow I feel like there is no particular

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thing that I'm working but I feel like I'm working on post translation modification.

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But thing is that even I feel like when I'm communicating and writing a grant for something

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I feel like where I should focus rather than like I don't have the you know niche where

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I can go and say like okay I want to work over there but it's a post translation modification

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which makes sometimes harder for people like other people also maybe thinking like we have

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moved in multiple projects that may be one of the reason that they're you know grant

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gain are not successful.

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Yeah I think I mean I think your grant has to be focused your proposal has to have you

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can't try and incorporate everything that you've done and it's fine to have developed

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some avenues of research that you've then not followed up on because you still learn

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from that.

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Yeah.

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But there should be it should build on something that you already know how to do that might

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be methodology you might apply existing methodology to a new system or you might continue to

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ask the similar questions to you've asked in your postdoc and take them in a slightly

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different direction.

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So it doesn't have to build on everything you might have done a PhD and a postdoc in

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different fields and it might only build on one of them not not on both of them.

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I think that's totally fine.

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That's right.

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So if I say I know postdoc is always like sometimes you know very hectic to cope up

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with all this like you have a two years of postdoc there's insecurity that after two

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years what will happen because of the your funding and all.

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So how do you deal with that from like if I have this year or two years I have to give

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this data but suddenly you don't know how much time it will take your research because

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it's like uncertainty in the world.

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Yeah I found the short term nature of it quite tricky at times and I think you've got to

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be really careful to prioritize the most important strands not the easiest wins.

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So it can be tempting to say oh this experiment will only take me two days to get the results

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I'll do that and then you do another experiment and another one.

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But actually sometimes the most important thing that actually going to be key for the

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paper you put it off but that might take eight months to do that so you need to get that

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started.

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But I think all career stages now I've got my own independent position you still feel

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like you're juggling a lot of things and you're prioritizing and there's a lot still a lot

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of pressure and time frames to get things done and get funding in so that doesn't go

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away it just evolves.

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So I think that pressure time of the postdoc is kind of teaching you how to deal with those

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pressures and how to manage multiple threads at once and how to prioritize.

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You do need to be able to do that to a certain extent to run a research group.

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So it's totally different as a postdoc and as an independent PI because in the independent

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you have to take care of your projects your tenure to complete and all those things but

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as a postdoc we have different perspective because I'm a postdoc I'm focusing on that

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because I don't know what will be there.

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I think the pressures evolve and are different but certainly with the postdoc it's the instability

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I think is the hardest bit because if you don't get stuff through to publication it

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can be quite challenging and I mean I'm still trying to push out a publication from my postdoc

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because I didn't get it finished within the postdoc and then it's hard to find time and

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it's frustrating for me how long that's taken it's frustrating for my PI and at least how

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long that's taken and we're really trying to push it out and I'm afraid there's no easy

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fix for it it's just hard but I think real careful prioritization of what is going to

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get me what's the minimum I need to get a paper out of this do that get the paper kind

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of get an output.

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Yeah prioritize the things what is needed not other than exploring every aspect and

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you are ending with nothing in your hand.

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Don't save everything up for that hypothetical nature paper obviously if you've got a really

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big story you might want to try for that big impact journal.

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But at the same time I feel like at the same time when you're aiming for high papers but

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you have to be realistic upon your work what you're doing because sometimes you can't say

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this paper is going to be in the highest impact journal you have to be open up with like okay

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if I'm doing these things if it's a novelty let's try it but sometimes you can't say okay

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I can stay for more one year I could try that can drive like a three or four years you're

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ending with nothing.

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I mean you know not the high impact but four years spend you got one so somehow sometimes

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feel like as we spend more time in the postdoc and our publication drops that's also negative

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for the career like in the future if you're applying for some kind of independent philosophy

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because in three years what you got is like this impact factor.

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Yeah I think there's a real balance and I think it's quite damaging for science the

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obsession with impact factor that we've generated and it doesn't lead I think to the best science.

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Best practices yeah that's right.

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But I do think there's different scales of impact and you do need your publications to

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go to a good rigorous journal that's respected in your field but I think it's far more useful

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for your career to have a slightly smaller paper but that you very clearly lead on than

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maybe be one of many authors on a huge paper because you're one of the big things about

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transitioning from the postdoc stage into an independent stage is being able to demonstrate

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your independence and your leadership and that's very hard you know no one gets a nature

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paper on their own those papers have you know it can become quite hard to demonstrate your

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independence and your contribution.

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And certainly sometimes it may help you as a teamwork you can say like you can work in

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a team that's good for like postdoc because you can work in multiple projects because

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when you join a lab you don't have to work on one project rather than you have to supervise

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sometimes PhD sometimes master and something like that so that that can define like you

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can work in a team but ultimately like you said small paper also matter because you lead

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them by yourself and you come to that one and small paper again can add multiple things

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like and finally you can say that you can lead the big project as well because as a

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career we cannot apply for the big grant first of all but like a small grant we can apply

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like for me like I'm looking for like a next one what will be either independent fellowship

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which I can take it because when I apply for the postdoc now I spent three or four years

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here in Durham and I apply for the job which doesn't have the criteria of my salary according

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to that and I feel like they won't be taking because they have mentioned this one and like

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this one the salary range so I sometimes feel like either you have to approach in such a

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way either independent philosophy be applied for together so that will be more entertaining

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rather than I will go for like open post everywhere they have means and already like we have this

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range so I feel what do you suggest.

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I think that's a very yeah kind of a very personal decision but certainly the independent

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fellowship gives you a bit more freedom to set your own parameters for every aspect of

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the job you kind of have a little bit more freedom to set your salary although still

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within institutional boundaries and you've got a lot more freedom on the direction of

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the project but it is a lot of work to put a fellowship together and obviously the success

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rate is low because they're very competitive so there's pros and cons I think if you can

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get a fellowship they're fantastic they really give you time to develop your ideas and your

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independence while you don't have say as a lecturer you've got teaching constraints

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as well but you don't have that so much as a as an early career fellow so if you can

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get them they're amazing but obviously they're competitive and there's plenty of brilliant

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scientists that don't get independent fellowships and I think it's important that they still

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try and go for for lecturer jobs and things because you don't need a fellowship to move

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into a lecturer position yeah plenty of people still don't.

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Yeah so recently I was applying for like a assistant professor in India and they have

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criteria for different like if you're applying for assistant professor you have to be like

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master or PhD no more experience but associate professor maybe five years after PhD you have

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to have experience of this one or industrial experience or teaching experience I came to

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like teaching experience in UK I don't know how many postdocs go for teaching because

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they don't allow sometimes like you have to work so somehow you're not developing yourself

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as a looking for like assistant professor or lecturer or something because they will

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ask do you have experience but no because in the PhD you just went for demonstrating

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and experiment not like a teaching on so I feel like that's missing in.

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Yeah I think that's a very good point and I should say my experience is obviously is

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very UK centric because I've only worked in the UK system and I've got family ties here

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so I'm not planning to move outside the UK system so it's not something I'd considered

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because if you're a postdoc applying for lecturer jobs in the UK there's no real expectation

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that you've done a significant amount of teaching because that's what the system in the UK is.

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It's a challenge for kind of other job markets that have different expectations and yeah

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I hadn't considered I think there are opportunities at postdoc to teach often for example I don't

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know what it's like here in Sheffield we have modules that have got like teaching focused

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stuff on them but then we have research focused guest lectures in those modules to give students

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exposure to kind of research led stuff and we sometimes have postdocs delivering those

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and so then you've got kind of examples of giving lectures and running practicals and

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things like that.

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Yeah that's really wonderful because here I feel like PhD scholars can opt for the demonstrator

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because they can be paid for that one and that's good but as a postdoc sometimes we

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feel like we haven't got any chances for like you know interaction with the undergrad or

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like a teaching experience you can include but sometimes like if there is a module where

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you can give a seminar so we approach over there they will be not paying for that but

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at least we are getting the experience for like interaction with the students that can

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may help you in later because suddenly when I am applying for this job I can say at least

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yeah I have worked for with a master and all but not completely like a lectureship or something

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like for one month or something but there is a one or two class I have taken for this

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one.

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Yeah I think giving a couple of guest lectures here or there gives you you can spin that

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to be quite a lot on your CV if you need to.

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So I will come back to you like as an independent researcher how do you unwind yourself when

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you are working in the lab or the office and applying for grants and all how do you unwind

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yourself?

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So I mean I have a two year old at home so there is not a lot of relaxing but because

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she is manic but I try and be quite boundaried and I think I try and not to let work bleed

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into personal life too much sometimes you do have to I do have to do a little bit of

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work in the evening and the weekends but for the most part my work day is when I work and

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then when I go home my laptop stays at work or it is in my bag and I do not think about

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it and I tell people in my lab that they will not get any response from me over the weekend

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but I also will not be emailing them over the weekend.

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And yeah try not to think about work in a work way I think about science still because

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I am interested in science but yeah spend time with my family and kind of have the weekend

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to do whatever I want to do go and see friends I have got an allotment I go and garden take

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time away from it and also in work I try and give myself time to think so if you are constantly

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doing little jobs here and there you never think and I sometimes just try and give myself

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an hour where I will go and sit in a coffee shop and just with a notebook and just take

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some notes think about work with no computer so you are not distracted by emails or finishing

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of a job just sketching out ideas but crucially that is work so that happens in the work day

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I am thinking and I am planning so it is a work job so it happens in the work day.

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That's right.

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I also like you said I can't carry this notebook with me if I am distracted too much with my

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work because whenever I am looking at my laptop I start doing like oh I have to work this

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one then I realise oh I have to apply for my post-op position so all the time I am like

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switching from one to another so and it is like you can do it because you are just typing

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like oh post-op position in the UK you are moving but you are not focusing and one hour

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I will spend and I will be like too much distressed so I feel like you said go to coffee shop

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go to I mostly go to the drum is really nice place you know it is like a nice garden you

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can go and just sit over there and just listen to the music and relax so yeah that is a good

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one.

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There is a lot of evidence about how constant task switching basically reduces how much

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you are able to focus on a thing and so every time you task switch you are losing focus

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and you are not regaining that when you switch back and so you lose productivity so it is

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better to try and carve out kind of bits of time for those sorts of important jobs without

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being distracted.

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If I ask you like how is it different now because as a post-doc all the time I am thinking

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about my work it is like you know you have to bring that work before your contract ends

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but as a PI you know that you have to carry on but you have to also focus on your personal

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life and you know work balance something like that but is it different like how you did

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when you were doing post-doc?

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I always tried to be quite bound read I feel like now that I kind of lead people in a lab

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I try and set a good example of having work life balance because I feel like those sorts

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of things have to come from the top if your PI works all the time and is always in it

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the expectation bleeds onto your post-docs and PhDs and I do not have that expectation

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of them.

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I think I am still thinking about stuff a lot of the time and it is just what I think

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about different before I was thinking about finishing that experiment now I am thinking

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about do I have time to go into the lab I have to write that lecture, shall I submit

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a grant but I am also thinking about trying to keep on top of the PhD students project

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so that when I speak to them I know what they are doing and they do not feel like I am out

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of touch with their project.

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It is better and worse there is more things to think about but then also I know that the

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students are doing some of the thinking as well because they are managing their own project

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to a certain extent so yeah it is kind of similar but with nuances I would say.

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So how do you explain because I was working with the research culture so we were talking

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about what kind of you know what do you think about the research culture according to your

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personal view because as a work ethic most of the time we are like okay you are working

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on so sometimes you spend extra time Saturday Sunday people are working day and night that

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is good for some time because this is demanding for this week only not for like a whole year

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how do you think about this toxic culture or something?

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I think it is very very hit and miss I think different places but also within a place different

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people have healthier or less healthy attitudes and I think it is a reasonable expectation

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with the way science works that it is not always nine to five but if someone has got

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an experiment that say runs late or runs over the weekend I think it is a perfectly fair

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expectation that you take that time back the following week or the next month or whenever

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time lets up and what you cannot allow to happen is that feeling of busy busy busy

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busy to elongate and be content especially when you have got PhDs and postdocs that you

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are managing as a manager I think it is really it is the PI's responsibility to manage those

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pressures and to take some of that pressure so when I was working with Jocelyn in Leeds

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we had a meeting and she said to me once she is like I think you are I can see that you

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are stretched too thin I think you are stretching yourself too far and you are going to burn

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yourself out we need to rejig this work load and she had that kind of and initially I did

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not respond very well because I felt like she was criticising me she was not she was

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protecting me and she was saying no I can see that you are doing too many things and

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you are not having time to rest I need to take some off you and that is I think that

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is the PI's job and I think some PI's will naturally do that better than others and I

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think it is important that institutions have a set of minimum expectations that they enforce

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with their PI's and there is clear pathways for people in those groups if those expectations

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are not being met to raise them but I think there are lots of there is lots of progress

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on research culture certainly in the UK but there is still also plenty of negatives but

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I think it is often overlooked that there is lots of negatives in other fields as well

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I have friends that work in the kind of the tech sector and there is all sorts of toxic

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cultures there not saying that is an excuse but I think sometimes academia is maybe unfairly

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demonised for not being better than other jobs when it is actually just the same as

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other jobs.

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Yeah that is right.

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We are coming to the end of this podcast however I will ask if you have any suggestions for

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the undergrad or postdoc or as an early career scientist what do you give them?

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I would say don't expect everything to run smoothly and to work certainly when you get

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into kind of lab projects and research projects stuff goes wrong or you don't get results

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that you expect or you get conflicting results and if you get disheartened by that that is

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going to happen throughout your whole career you have really got to learn to manage that

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and be kind of resilient and sometimes that might turn into a win sometimes that is just

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a bad avenue and you have to change tack but learning to deal with that I think gives you

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the resilience to carry on.

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Yeah most of the time I am saying my personal view because when I work on something I put

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myself in that work and suddenly you realise it is not working and I am spending one month

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or six months.

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Sometimes I feel like it is like my personal view like is it worth to come to the science

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like it is like you are questioning yourself and sometimes you don't find answer or support

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from other people because there is things sometimes you can't discuss with the people

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even your friend because they will oh Prakash come on you are not doing enough maybe people

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think like that way right so you know how do you going to suggest like if you come to

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this point I suggest myself like you are doing science you know that it won't be hard first

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thing you won't be getting the experiment one day and you get the data you will be happy

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but stick over there but sometimes I feel very negative for myself it's like.

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Yeah I think that's tough and there is a lot of imposter syndrome in science where people

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that are really good think that they are not very good but if you are in industry industry

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would have lots of checkpoints where they say right if we have not achieved this by

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then we can't proceed if we have not achieved this by then or we need these to work for

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this to be a viable avenue and I think sometimes in academia we get a little bit too emotionally

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attached to the work because we find it fascinating and it's cool and you want to address the

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question but it's not always a good use of mental resources and I think building in the

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checkpoints early so during my PhD I had a big AFM atomic force microscopy project and

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it was really challenging and there was an element of it that we ended up abandoning

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but we decided before we even started it we were like right if we have not achieved this

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by this point we are going to move on because we don't have time so it means when we hadn't

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it wasn't like a shock to me because I knew that was what we were building to and I knew

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we had an end point as well so I think if we built those in it makes it a little bit

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easier but it's always hard to let go of a bit of a research project that you feel a

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lot of affection but sometimes it's for the best and an experiment not working isn't a

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reflection on the researchers ability sometimes experiments are just not tracked.

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Or the hypothesis is wrong but it was the best hypothesis of the time or we don't have

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the technology yet maybe in 10 years there'll be a new tool and that'll be easier.

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Yeah that's right.

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Alright so thank you Sam for coming to our podcast.

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Before ending can you say something for your group if somebody wants to join your group

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as a PhD or you know postdoc, say anything?

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Well actually yeah I'll be looking for PhD students to work on well there's a bit of

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flexibility actually but around cell wall development in plants or fungi and the role

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of that in pathogenicity and in stress responses and I'll be looking at that towards the end

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of this year looking for students so if people are interested for PhD it'll be on my Twitter

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which is at Sam Amesbury and I'm hoping to get postdoc funding soon and so again postdoc

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will be advertised through the same.

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So thank you so much Sam for coming and all the best for your career.

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Thank you for having me.

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Thank you.

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Wow.

